Faculty Advisor

Rao, Reeta Prusty

Abstract

Candida infections are common opportunistic infections among humans, especially those with an underlying immunodeficiency. Five species of Candida are most often seen clinically and these include Candida tropicalis, Candida krusei, Candida glabrata, Candida dubliniensis, and Candida albicans. There is growing concern about the emergence of antimicrobial resistant and often misdiagnosed species of Candida albicans and Candida dubliniensis. This study will lay the foundation to study the mycobiome and differentiate between the species of Candida. Clinical isolates were inoculated on CHROMagar for phenotypic pre-identification after 48 h of incubation at 37 °C and genotyped by PCR using species-specific primers ITS regions followed by a PCR-RFLP digestion.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2016

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Share

COinS