Faculty Advisor

Shell, Scarlet


This project was designed to study the ability of Mycobacterium smegmatis to survive hypoxia. This project focused on the msmeg_3213 gene in M. smegmatis, which is predicted to code for the DNA adenine methyltransferase MamA. The goal of this project was to determine the function of DNA methylation in M. smegmatis. A knockout was designed and built using PCR and Gibson Assembly. The knockout plasmid was transformed into E. coli to clone it; however, no correct clones were obtained. If cloning had been successful, the plasmid would have been purified, and transformed into M. smegmatis to remove the gene. If I had been able to successfully delete the msmeg_3213 gene from M. smemgatis, the viability of the transformed bacteria would have been tested in both hypoxic and normal environments.


Worcester Polytechnic Institute

Date Accepted

May 2016


Biology and Biotechnology

Project Type

Major Qualifying Project



Advisor Department

Biology and Biotechnology