This report outlines the implementation and proof of concept testing of dual-optical-fiber optical tweezers in studying cell properties. Customized equipment was created to enable testing. This equipment included an aluminum platform to stage equipment that otherwise could not align with the microscope and a 3D stage assemble to allow precise positioning of optical fibers and other equipment near the target sample cells. A method for applying 5 um diameter silica beads to live 3T3 fibroblast cells was developed using a 20 um diameter tip micropipette. These microbeads were manipulated on the cells using the fiber optic tweezers to demonstrate the feasibility of using such a setup to measure cell mechanical stiffness.
Worcester Polytechnic Institute
Major Qualifying Project
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