This research investigated possible predictive biomarkers for embryo yield of SE in Pinus taeda suspension culture. Previous research in related systems suggest that AGP levels or culture size may serve as a determinant for embryo yield. The culture was fractionated with a 710 um sieve and the embryo yield of each fraction was determined 12 weeks after plating on developmental media. Cell-associated AGP concentrations in each size fraction were measured to determine if there is a difference between large and small aggregates. Results demonstrated that the >710 um fraction developed more embryos; additional size verification is recommended to confirm this relationship. Results also showed that larger aggregates have higher concentrations of AGP than smaller aggregates.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Hague, Nathan Daniel
• Bodanza, Michael D.

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042518-210632

Advisor

• Roberts, Susan Celia

Year

• 2018

Date created

• 2018-04-25

Resource type

• Major Qualifying Project

Major

• Chemical Engineering

Rights statement

• In Copyright

Last modified

• 2020-11-14