Faculty Advisor
Weathers, Pamela
Abstract
This study explored the role of hypermethylation of DNA in transformed hairy roots of Artemisia annua in diminishing production of artemisinin when maintained in culture for long periods. Two weeks following treatment with 5-azacytidine roots showed inhibited growth, but no change in artemisinin production. HPLC was used to analyze artemisinin content. DNA in treated showed reduced methylation compared to controls when compared through TLC and quantitated using spectroscopy. Analysis of the mitotic figures in the root tips indicated that 5-azacytidine inhibits growth by arresting cell division in metaphase, preventing elongation of the roots.
Publisher
Worcester Polytechnic Institute
Date Accepted
April 2007
Major
Biochemistry
Project Type
Major Qualifying Project
Copyright Statement
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Accessibility
Unrestricted
Advisor Department
Biology and Biotechnology