Faculty Advisor

Heilman, Destin

Faculty Advisor

Wobbe, Kristin K

Abstract

To facilitate studies of HTLV-1 protein Tax interaction with the APC/C activator protein Cdc20 in S. cerevisiae, a 9 amino acid hemagglutinin epitope tag was attached to the C terminus of the Cdc20 protein using homologous recombination of the yeast genome. A PCR product was engineered to contain homologous arms that would facilitate recombination of the tag, as well as a TRP1 marker, into the cdc20 locus. Integrants were selected on Trp- media and positive clones were identified using western blotting.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2008

Major

Biology and Biotechnology

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Chemistry and Biochemistry

Advisor Department

BC

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