Faculty Advisor

Flaherty, Patrick J.

Faculty Advisor

Vidali, Luis

Abstract

To identify genes important for growth, we generated conditional mutants that experience loss of growth at high temperature (CLoG mutants). We outcrossed one of these mutants, CLoG4, to a polymorphic strain and sequenced the whole genome of a pool of segregants that maintained temperature sensitivity to map the mutation causing the phenotype. I also performed a rescue experiment to confirm the identity of the phenotype-causing mutation of a second strain, CLoG1, which had already been mapped.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2015

Major

Bioinformatics and Computational Biology

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biomedical Engineering

Advisor Department

Biology and Biotechnology

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