Isolation of Temperature-sensitive Mutants in the Moss Physcomitrella patens and Mapping of their Causal Mutation by Genome Sequencing of Pooled Segregants

Ding, Xinxin

Student Work

Isolation of Temperature-sensitive Mutants in the Moss Physcomitrella patens and Mapping of their Causal Mutation by Genome Sequencing of Pooled Segregants

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To use plants to achieve a sustainable future, it is critical to understand how plants grow, specifically how genes direct cell growth and cell division. One effective approach to investigate these genes is the isolation of conditional mutants. This project used the moss Physcomitrella patens as the model organism and focused on isolating temperature sensitive mutants with impaired growth, characterizing their morphology under the restrictive condition, as well as trying to identify the genetic mutation that causes the temperature-sensitive phenotype.

This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

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