Faculty Advisor

Arguello, Jose' M.

Abstract

Transition metal homeostasis is important for organisms, as they are both essential for biological processes as well as toxic. The opportunistic parasite Pseudomonas aeruginosa has shown that metal ion homeostasis, specifically copper, may be important to its pathogenesis. This study utilizes the nematode Caenorhabditis elegans as a model organism to study this pathogenesis. C. elegans was exposed to mutants of P. aeruginosa that pertain to the bacteria’s copper homeostasis system and lethality was measured over time to infer the importance of these genes to P. aeruginosa’s pathogenesis. Here, it is shown that knockouts of these genes do cause a decrease in lethality, suggesting copper homeostasis is an important measure by which P. aeruginosa resists the immune responses of its hosts.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2018

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Chemistry and Biochemistry

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