Faculty Advisor

Roberts, Louis Anthony

Faculty Advisor

Shell, Scarlet

Abstract

Tuberculosis, caused by Mycobacterium tuberculosis, is a highly infectious disease. TB is unique in that it can survive stress in the human body, including hypoxia. A possible survival mechanism is the influence of 5' UTRs on gene regulation. To test this, a series of constructs were built to include a strong promoter and a fluorescent reporter gene. One construct introduced a degradation tag; another removed the MOP associated 5’ UTR. Constructs were assessed by fluorescence microscopy and colony color to evaluate mCherry expression. Findings revealed that the degradation tag was too efficient and the UTR was unnecessary. The final construct contains the MOP promoter and the mCherry reporter gene and can be used in future studies to test the impact of various 5’ UTRs on gene expression.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2018

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Share

COinS