Faculty Advisor

Ryder, Elizabeth F.

Center

University of Massachusetts Medical Center

Abstract

Two chromatin remodeling factors Mbd3 and Brg1 affect the pluripotency in mouse embryonic stem cells. KD of the two factors showed that they oppositely affect a common set of genes. Genome wide mapping of Mbd3 and Brg1 using ChIP-Seq showed that the two factors preferentially bind to promoter regions and physically interact in ES cells. Mbd3 binding is found to be enriched at Polycomb and bivalent target genes marked with H3K4me3 and H3K27me3.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2011

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Project Center

University of Massachusetts Medical Center

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