Faculty Advisor

Rulfs, Jill

Abstract

Two monosaccharide labeling agents, 1-phenyl-3-methyl-5-pyrazolone (PMP), and 4-(3-Methyl-5-oxo-2-pyrazolin-1-yl) Benzoic Acid (PMPA), were investigated to determine monosaccharide content in palm oil mill effluent waste (POME). Methods published in the literature were optimized to increase efficiency of monosaccharide detection to suit industry needs. The results from PMP derivatization, using pH 8.0 PBS for dilution and microwave irradiation suggest this is the most efficient derivatization method tested in this project. Monosaccharides detected in POME samples were mannose, glucose and xylose.

Publisher

Worcester Polytechnic Institute

Date Accepted

December 2007

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

BC

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