Faculty Advisor

Adams, David S.

Abstract

Two primary differences between epithelial cells and cancerous cells are the ability of cancerous cells to metastasize and avoid apoptosis. The drug 4-methylthio-2-oxobutyric acid (MTOB) has been shown to interact with the CtBP transcription repressor causing the induction of apoptosis, as well as a reduction in migration of cells. The focus of this MQP was to explore the most efficacious use of MTOB through the study of structural analogs, as well as combination therapy with CtBP siRNA. While the structural analogs used were less effective than MTOB, using siRNA against CtBP2 in combination with MTOB proved to significantly lower cell growth rate and increase apoptosis. Immunoblotting analysis demonstrated a decrease in expression of the CtBP2 protein with the combination treatment.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2010

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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