Faculty Advisor

Adams, David S.

Center

UMMC / University of Massachusetts Medical Center

Abstract

Typically, new neurons migrate randomly out of the RMS into the olfactory bulb. This experiment seeks to view the effect of the release of GABA from active granule cells, which is hypothesized to cause directed migration of neurons in the olfactory bulb. Mouse granule cells were experimentally altered through use of lentiviral vectors delivering genes for ion channel Nachbac. Then, fluorescence microscopy, and the Neurolucida computer module were used to investigate the behavior and distribution of new neurons in the olfactory bulb. Data analysis and experiments in the UMASS Lab show that experimental group TRPA1 works to silence granule cell activity. It is shown that TRPA1-treated GCs work to repel new neurons from the area.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2013

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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