Faculty Advisor

Adams, David S.

Abstract

TYRA-3 is a tyramine-activated G-protein coupled receptor in C. elegans. TYRA-3 is expressed in dopaminergic ADE and CEP neurons, suggesting it may play a role in modulating dopaminergic signaling. Upon exogenous tyramine exposure, deletion mutants of tyra-3 are more susceptible to paralysis than wild type animals. dat-1 deletion mutants, which contain abnormally high concentrations of synaptic dopamine, were also sensitive to paralysis with tyramine exposure. cat-2 deletion mutants, which contain no dopamine, were partially resistant to tyramine exposure. Double mutants of cat-2;tyra-3 and dat-1;tyra-3 showed moderate sensitivity. These results suggest that TYRA-3 inhibits dopamine release, but additional experiments are necessary to explain the phenotypes of the double mutants.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2010

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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