Upon cellular differentiation the late genes of the Human Papillomavirus (HPV) are expressed. Recently, the CTCF DNA-binding protein has been found to be transcription factor and chromatin insulator. We examined if CTCF plays a role in viral transcription and late gene expression. Through ChIP analysis it was found that CTCF binds to two distinct regions of the Bovine Papillomavirus-1 genome. It was observed that the E2 protein and CTCF protein interact by co-immunoprecipitation. Using RNA interference, the effect of CTCF on viral transcription was examined. It was shown that E2 and L1 RNA levels are knocked out with lower levels of CTCF. Further studies should investigate if the type of interactions between E2 and CTCF, and the mechanism in which CTCF controls viral transcription.
Worcester Polytechnic Institute
Major Qualifying Project
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Chemistry and Biochemistry