Faculty Advisor

Rulfs, Jill

Abstract

V. cholerae colonizes the human intestine via TCP & can cause diarrhea with fluid losses severe enough to be fatal. Without TCP, V. cholerae cannot attach to the intestine, secrete toxins, or cause GI disease. 4 proteins required for TCP biogenesis -- TcpD,E,R,& T -- localize to the IM. Previous experiments support the hypothesis that interactions among 3 of these 4 proteins are predicted to be sufficient to form a stable IM platform in V. cholerae. Plasmids were engineered to carry combinations of tcp genes and induced to co-express the 4 IM Tcp proteins in all groups of 2 & 3 in E. coli. Protein immunoblots on cell lysates suggest that TcpD and TcpE stabilize the TcpR-TcpT interaction. Therefore, 3 proteins required for TCP biogenesis appear to form a stable IM platform.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2007

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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