We analyzed the ability of bone marrow-derived stem cells, umbilical cord blood-derived stem cells, quiescent, and FGF-2 stimulated adult human dermal fibroblasts to differentiate into the three main lineages. Histochemical and immunocytochemistry assays were performed on the cell cultures to determine the success of specific differentiation protocols. The data showed that all treatments except the umbilical cord blood-derived stem cells differentiated into the adipogenic lineage, although none of the treatments successfully differentiated into the chondrogenic or osteogenic lineages. The purpose of this project was to develop in vitro assays with which to measure success of future de-differentiation experiments.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Whitton, Holly Jane

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042208-184816

Advisor

• Page, Raymond L.

Year

• 2008

Date created

• 2008-04-22

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright