Faculty Advisor

Page, Raymond L.

Abstract

We analyzed the ability of bone marrow-derived stem cells, umbilical cord blood-derived stem cells, quiescent, and FGF-2 stimulated adult human dermal fibroblasts to differentiate into the three main lineages. Histochemical and immunocytochemistry assays were performed on the cell cultures to determine the success of specific differentiation protocols. The data showed that all treatments except the umbilical cord blood-derived stem cells differentiated into the adipogenic lineage, although none of the treatments successfully differentiated into the chondrogenic or osteogenic lineages. The purpose of this project was to develop in vitro assays with which to measure success of future de-differentiation experiments.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2008

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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