Faculty Advisor

Adams, David S.

Center

University of Massachusetts Medical Center

Abstract

When treated with interferon-beta-1b, gene NCOA7 expresses a new variant that begins from an alternative start site NCAO7-AS with strong antioxidant properties. Segments of three different lengths of the upstream region of the gene were inserted into phRLnull. After testing and confirming their structure, the plasmids were transfected into mammalian cells. A luciferase assay was performed to determine which segments showed an increase in activity when treated with interferon. While each ligated plasmid acted as a strong promoter for Renilla luciferase, no significant difference in signal due to INF-beta- 1b treatment was observed.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2013

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Project Center

University of Massachusetts Medical Center

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