Faculty Advisor

Prusty Rao, Reeta

Abstract

While ethanol holds promise as a fuel source, implementation is hampered due to reliance on material used in food production. Lignocellulose, an abundant, unexploited substrate is problematic due to the inability of microbes to ferment it. Here a novel approach for Saccharomyces cerevisiae metabolic engineering was employed, using mutagenesis of a TATA-binding protein combined with traditional genetic engineering to produce a phenotype capable of lignocellulose metabolism. This promises a means of creating desired ethanol producing phenotypes. We report success engineering a new yeast strain, containing a desired genotype for metabolic engineering, as well as a strain that can successfully grow in a culture having only xylose and arabinose.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2009

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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