Faculty Advisor

Adams, David S.

Faculty Advisor

Scarlata, Suzanne Frances

Abstract

While Phospholipase-CB1 (PLCB1) plays an important role on the plasma membrane by generating calcium signals, it also has a significant population in the cytosol where it shows affinity for Component 3 Promoter of RISC (C3PO), which is an enhancer of RNA silencing. This project focused on PLCB1’s inhibition of RNA silencing in vitro and in PC12 cells by evaluating the hydrolysis of fluorescent oligonucleotides. We find that PLCB1 binds and inhibits C3PO, allowing the expression of genes whose sequence would otherwise be degraded.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2018

Major

Interdisciplinary

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Advisor Department

Chemistry and Biochemistry

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