Adams, David S.
Scarlata, Suzanne Frances
While Phospholipase-CB1 (PLCB1) plays an important role on the plasma membrane by generating calcium signals, it also has a significant population in the cytosol where it shows affinity for Component 3 Promoter of RISC (C3PO), which is an enhancer of RNA silencing. This project focused on PLCB1Âs inhibition of RNA silencing in vitro and in PC12 cells by evaluating the hydrolysis of fluorescent oligonucleotides. We find that PLCB1 binds and inhibits C3PO, allowing the expression of genes whose sequence would otherwise be degraded.
Worcester Polytechnic Institute
Major Qualifying Project
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Biology and Biotechnology
Chemistry and Biochemistry