Faculty Advisor

Dilorio, Alexander A.

Abstract

-N-acetylgalactosaminidase is an enzyme that has several implicated applications in the biotechnology industry. In its productions from E. coli, there is an unknown inhibitor that is also co-produced. The goal of this project was to characterize and purify this inhibitor in hopes of the development of a more efficient means of purifying -N-acetylgalactosaminidase in the future. Many techniques for purification were used such as: Tangential Flow Filtration (TFF), Cation Exchange (CEX), Aqueous two-phase extraction( ATPE), Ammonium Sulfate precipitation, Hydrophobic Interaction Columns (HIC), as well as enzyme kinetic studies and inhibition kinetics.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2004

Major

Biology

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Restricted-WPI community only

Advisor Department

Biology and Biotechnology

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