Faculty Advisor

Rulfs, Jill

Abstract

-Polyglutamic Acid has the potential to be used as a slow release drug delivery system. PGA conjugated to insulin was administered to insulin starved Reuber H35 rat hepatoma cells, and by GSK-3 phosphorylation, was shown to mimic physiological insulin. Through time course and dose response experiments we determined that 10M PGA-insulin and 25 minutes showed the maximum GSK-3 phosphorylation. Receptor ligand crosslinking was performed to determine if insulin bound to the receptor retained glutamic acid residues from the polymer.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2003

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Restricted-WPI community only

Advisor Department

Biology and Biotechnology

Advisor Program

Biology and Biotechnology

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