A focal adhesion targeting sequence within the protein supervillin regulates the turnover of focal adhesions and its associated actin-rich stress fibers. The 1000 Genomes Project identifies naturally occurring human variations within this sequence. Point mutagenesis was undertaken to determine if these human variations affect stress fiber formation in transfected COS7-2 mammalian cells. The data suggest that hSV down-regulates stress fiber counts along with the naturally occurring variant of G466R; whereas RY/AA produces results similar to those depicted in bSV, and T426A increases stress fiber counts.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Yusif, Michelangela Amira

Contributors

• Luna, Elizabeth

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042513-163120

Advisor

• Adams, David S.
• Heilman, Destin

Year

• 2013

Sponsor

• UMass Medical School

Date created

• 2013-04-25

Resource type

• Major Qualifying Project

Major

• Interdisciplinary

Rights statement

• In Copyright

Last modified

• 2023-09-20