Faculty Advisor

Adams, David S.

Faculty Advisor

Heilman, Destin

Center

University of Massachusetts Medical Center

Abstract

A focal adhesion targeting sequence within the protein supervillin regulates the turnover of focal adhesions and its associated actin-rich stress fibers. The 1000 Genomes Project identifies naturally occurring human variations within this sequence. Point mutagenesis was undertaken to determine if these human variations affect stress fiber formation in transfected COS7-2 mammalian cells. The data suggest that hSV down-regulates stress fiber counts along with the naturally occurring variant of G466R; whereas RY/AA produces results similar to those depicted in bSV, and T426A increases stress fiber counts.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2013

Major

Biology and Biotechnology

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Advisor Department

Chemistry and Biochemistry

Project Center

University of Massachusetts Medical Center

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