Faculty Advisor

Dominko, Tanja

Abstract

Chromosomal mutations lead to abnormal karyotypes and multiple genetic disorders in a variety of mammalian species. During extended cell culture and proliferation, human stem cells have demonstrated a predisposition to deletions or amplifications of multiple regions. Here we describe two additional chromosomal abnormalities (deletions on Ch10 and loss of heterozygosity on Ch5) associated with a female human embryonic stem cell H9 line that failed to undergo induced differentiation. Cytogenetic studies detected amplifications, deletions and translocations in multiple chromosomes. In an attempt to characterize these mutations, remapping, sequencing, and gene ontology was performed. Here we identify and analyze protein coding genes localizing to mutated regions.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2017

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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