Faculty Advisor

Weathers, Pamela J.

Abstract

Artemisinin (AN), a sesquiterpene found in the plant Artemisia annua, has been shown to have a potent antimalarial activity capable of killing malaria's infectious agent, Plasmodium falciparum. Artemisinin is produced via cyclization of IPP units produced in the terpenoid biosynthetic pathway by two branches: the mevalonate (MVA) pathway localized in the cytosol, and the non-mevalonate (MEP) pathway localized in the plastid. The regulatory enzymes of MVA and MEP pathway, HMGR and DXR respectively, have been shown to be present in the transformed roots of A. annua. By independent inhibition of HMGR by mevinolin, and DXR by fosmidomycin, artemininin yield was correlated to IPP contribution by one branch or the other. Artemisinin appears to be produced by a cooperative scheme of both the MVA and MEP branches.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2003

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Restricted-WPI community only

Advisor Department

Biology and Biotechnology

Advisor Program

Biology and Biotechnology

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