Faculty Advisor

Weathers, Pamela J.

Abstract

Artemisinin, the antimalarial compound, is not found in the culture medium of hairy roots of Artemisia annua L. that produce the terpenoid in vivo. Peroxidases in the medium are likely degradative agents. Three peroxidases were purified and concentrated from culture medium using dialysis and ion exchange chromatography. An anion exchange column with a pH gradient of 5.0 to 3.5 was used to elute the proteins. The three separated peroxidases will be used for future studies on the degradation of artemisinin.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2002

Major

Biology

Project Type

Major Qualifying Project

Accessibility

Restricted-WPI community only

Advisor Department

Biology and Biotechnology

Advisor Program

Biology and Biotechnology

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