Faculty Advisor

Dilorio, Alexander A.

Abstract

As a continuation of research begun on the feasibility of coupling expanded bed chromatography (EBC) and yeast fermentation the issue of enzymatic regulation was investigated. To simulate the nutrient starvation conditions of the EBC process, fed batch fermentations of Pichia pastoris were deprived of nutrients for similar retention times to those of an EBC column. Analysis of pre and post starvation Alcohol Oxidase (AOX) regulation in the form of mRNA production was used as an indicator as to the effects of the starvation and the overall feasibility and validity of the coupled process.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2007

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Restricted-WPI community only

Advisor Department

Biology and Biotechnology

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