Faculty Advisor

Adams, David S.


Biomarker discovery and proteomics are important for disease diagnosis and monitoring. This project evaluated the effect of protein digestion conditions and sample contamination on biomarker quantitation in biological mixtures cerebrospinal fluid (CSF) and plasma. Tandem mass spectrometry was used to analyze and identify specific marker peptides. Based on the data collected, the optimal method for peptide quantitation was dependent on the matrix tested, and varied between unbiased and targeted approaches. Blood contamination in CSF was shown to not be a major concern since only one of 56 targeted peptides was slighted affected.


Worcester Polytechnic Institute

Date Accepted

April 2017


Biology and Biotechnology

Project Type

Major Qualifying Project


Restricted-WPI community only

Advisor Department

Biology and Biotechnology

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