Hobey, William D.
Chloride dependent K cotransport channel in human erythrocytes was investigated, in attempts to further elucidate the mechanism of control in regulatory volume decrease activated by Ca? and propranolol. The effects of a phosphotyrosine phosphatase inhibitor, sodium orthovanadate and the protein kinase inhibitors, staurosporine and chelerythrine chloride were tested. Tests were conducted by mixing the inhibitor with washed erythrocytes, and buffered salline solution. A specialized computer program and apparatus measured the K efflux. Sodium orthovanadate inhibited K efflux at 1mM and 5mM; effects at higher concentrations were obscured by vanadate induced flux. Staurosporine and chelerythrine also inhibited K efflux. It was concluded that there is a protein kinase, possibly PKC, in the Ca?/propranolol pathway, and a phosphotyrosine further downstream.
Worcester Polytechnic Institute
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Chemistry and Biochemistry