Adams, David S.
Collagenase (MMP-1), stromelysin (MMP-3), and gelatinase (MMP-9) are three zinc-dependent metalloproteinases (MMPs) involved in joint destruction, and are particularly important in the pathogenesis of Rheumatoid Arthritis (RA). Ajulemic acid (Aa) and gamma-linolenic acid (GLA) are compounds that have shown therapeutic effects against RA, and which may reduce the production of the three MMPs. To verify this, Aa and GLA were added to cell cultures at different concentrations and then MMP expression was analyzed using ELISAs and northern blots. The results indicate that at a concentration 5 [mu]mol/ml Aa completely abolishes the enzymatic activity of MMP-9.
Worcester Polytechnic Institute
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Biology and Biotechnology