Faculty Advisor

Adams, David S.

Abstract

Compounds that stimulate interferon production in cells might have therapeutic effects against viruses that prevent the activation of interferon production. Our lab previously identified a compound as potential up-regulators of interferon production.. This project confirmed the effects of the compound and then analyzed the mechanism of interferon production using RT-PCR, bioassays, nanostring assays, and residual viral levels in vitro to determine the ability of the compounds to protect against viral infection. The results show that interferon production can be stimulated in a variety of cell lines (HEK293 cells, macrophages, dendritic cells, and fibroblast cells) and the protein RIG-I is necessary for interferon production by the compound.

Publisher

Worcester Polytechnic Institute

Date Accepted

March 2012

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Advisor Program

Biology and Biotechnology

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