Faculty Advisor

Adams, David S.

Abstract

Cyclin-dependent kinases (CDKs) are proteins that facilitate entrance of a cell through its cell cycle. Proteins p21 and p27 are CDK-inhibitors that arrest the cell cycle at the G0-G1 phase, and induce cell migration. The purpose of this MQP was to construct an expression system for biologically active p21 and p27. A TAT-mediated protein expression method was utilized for expression, and a HepG2 migration assay was developed to assay biological function. The data showed that recombinant proteins TAT-21 and TAT-27 were indeed biologically active in the migration assay.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2001

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Restricted-WPI community only

Advisor Department

Biology and Biotechnology

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