Faculty Advisor

Camesano, Terri A.

Abstract

Gene Therapy using polymers was studied to circumvent production and efficiency difficulties with viral and plasmid vectors. Polycationic polymers provide increased cell selectivity. One difficulty observed are formations of large aggregates. Aggregation properties of Polyethyleneimine (PEI) and Polynorbornene with Chlorine counter ion (Polynorbornene-C1) polymers were studied. UV spectrophotometry showed aggregates forming with ten minutes of contact for PEI, and within thirty minutes for Polynorbornene-C1. Positive zeta potential readings indicated both polymers neutralized DNA's negative charge. Scanning electron microscopy showed aggregates formed 0.1 um spheres.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2003

Major

Chemical Engineering

Project Type

Major Qualifying Project

Accessibility

Restricted-WPI community only

Advisor Department

Chemical Engineering

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