Faculty Advisor

Adams, David S.


GLUT4 trafficking is an integral process in insulin signaling. There are many different proteins involved, but a particular interesting protein is a substrate of Akt called AS160. AS160 was found to be insulin sensitive, being down-regulated in the cytosol, and up-regulated in low density microsomes. Working with Michael Czech at UMASS Medical Center, the focus of this MQP was to develop an antibody to mouse AS160 with a GST tag at the N-terminus. The antibody was then used with 3T3-L1 adipocytes as an in-vitro cell model to study the role of AS160 in GLUT4 trafficking.


Worcester Polytechnic Institute

Date Accepted

January 2003


Biology and Biotechnology

Project Type

Major Qualifying Project


Restricted-WPI community only

Advisor Department

Biology and Biotechnology