In 2016, cancer is expected to take more than 1,500 lives a day. Human Torque Teno Virus third viral protein (TTV-VP3) has been shown to induce apoptosis in cancer cells while leaving primary cells unharmed. To design a cancer therapy modeled after TTV- VP3, it is necessary to research its properties. The steady-state localization of TTV-VP3 has been shown to favor the cytoplasm. It is suspected that Leu35 and Leu36 are located in the putative NES that interacts with CRM1. Site-directed mutagenesis was performed to mutate these Leucines to Threonines in an attempt to hinder its export capabilities, and drive the steady-state levels of TTV-VP3 to the nucleus. It was determined that Leu35 and Leu36 are not essential for nuclear export but are essential for apoptosis induction.
Worcester Polytechnic Institute
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