Arguello, Jose' M.
Copper containing nitrite reductase (NirK) is a homotrimeric protein in Sinorhizobium meliloti makes use of six copper ions in total, but the molecular mechanism by which it procures its copper ions is yet to be determined. The focus of this project was to investigate the role of certain copper ATPases in the metallation of nitrite reductase. The wild type strain was explored, and the Griess method of nitrite determination was used to determine nitrite reductase activity and understand the relationship between nitrite reductase and copper ATPases. The methods that have been developed during this project can then be further refined and used in the future to determine copper ATPase metallation targets in S. meliloti.
Worcester Polytechnic Institute
Major Qualifying Project
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Chemistry and Biochemistry