Faculty Advisor

Adams, David S.

Abstract

In this project, the preliminary work for a study of nuclear import of HIV-1 in macrophages was conducted. RNA inhibition was used to prevent expression of karyopherins a1, a2, b2, and RCC1. siRNA techniques were tested and refined for each of the samples on HeLa cells by using real time RT-PCR to establish that mRNA inhibition occurred and performing Western blots to ascertain that specific cellular protein titers were lowered.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2003

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Restricted-WPI community only

Advisor Department

Biology and Biotechnology

Advisor Program

Biology and Biotechnology

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