Faculty Advisor

Adams, David S.

Abstract

Interactions between DNA repair proteins Rad51 and XRCC3 were explored to investigate their roles in homologous recombination with respect to breast cancer. Recombinant plasmids and mammalian cell lines containing various XRCC3 mutants were constructed for experimentation with yeast hybrid and MMC sensitivity assays to test XRCC3 interaction with Rad51 or Rad51C in repair. Our results confirmed physical interactions between XRCC3 and Rad 51 or Rad51C, respectively. Mutations within or near the ATP-binding domain of XRCC3 almost always completely disrupted interaction with Rad51.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2004

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Restricted-WPI community only

Advisor Department

Biology and Biotechnology

Advisor Program

Biology and Biotechnology

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