Fairchild, Craig D.
Light is essential for the growth of a plant. Phytochromes, a complex system of photoreceptors, are responsible for detecting light conditions and directing changes within the plant in response to changes in light. HFR1 and ATHB-2 are both involved in far-red light signal responses in Arabidopsis. The hfrl loss of function mutation is known to result in increased hypocotyl elongation when following exposure to continuous far-red light. ATHB-2 is upregulated by the hfrl loss of function mutation inducing shade avoidance, though the effect of an athb-2 loss of function mutant is unknown. F2 seedlings resulting from a cross of ATHB-2/ATHB-2 hfrl/hfrl x athb- 2/athb-2 HFR1/HFR1 were genotyped via PCR. The genotypes were then compared with hypocotyl lengths of the seedlings to determine the effect of the athb-2 mutation on hypocotyl lengths, specifically on hfrl mutant seedlings. Results showed no significant effect of the athb-2 mutation on seedling hypocotyl length.
Worcester Polytechnic Institute
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Chemistry and Biochemistry