Faculty Advisor

Rulfs, Jill

Abstract

Magnetic-activated cell sorting (MACS) was used to isolate and quantify antigen specific T cells. T cells specific to one vaccinia and three yellow fever peptides were isolated from two immune donors. Peptide specific cells were quantified by fluorescence-activated cell sorting analysis. Cytotoxic T lymphocyte (CTL) clones were generated from cultures obtained by the MACS assay through limiting dilutions. CTL assays on the bulk cultures and clones showed strong specific cytotoxic activity against target cells presenting their respective virus or peptide.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2000

Major

Biotechnology

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Restricted-WPI community only

Advisor Department

Biology and Biotechnology

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