Faculty Advisor

Ryder, Elizabeth F.

Abstract

Many genes are known to produce more than one form of mRNA by alternative splicing, in which exons are excluded from an mRNA transcript of a gene, thus altering the conformation of the protein. The dig-1 gene of the nematode Caenorhabditis elegans is involved in neuronal process guidance, and may undergo temporally mediated alternative spicing. Using RT-PCR analysis, two possible sites of alternative splicing were identified. Several RT-PCR products were cloned and sequenced to reveal the actual spicing patterns.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2003

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Restricted-WPI community only

Advisor Department

Biology and Biotechnology

Share

COinS