Faculty Advisor

Srinivasan, Jagan

Abstract

Maternal RNAs are transferred through the oocyte cytoplasm to the fertilized progeny. In metazoans, these transcripts play a critical role in patterning embryonic development prior to the maternal-to-zygotic transition. The 3'UTR appears to be the primary determinant controlling the pattern of maternal RNA expression in the germline and in the embryo. The 3’UTR also directs robust translation in male gametes, suggesting a possible role for mRNA inheritance through the male germline in C. elegans. Specifically, the set-2 3'UTR drives strong translation of a GFP reporter in sperm. This study aims on identifying cis acting elements in this UTR that confer strong sperm-specific translation, and screening for trans acting RNA binding proteins that influence sperm translation efficiency.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2017

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Restricted-WPI community only

Advisor Department

Biology and Biotechnology

Advisor Program

Biology and Biotechnology

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