Camesano, Terri A.
Hobey, William D.
Kazantzis, Nikolaos K.
Microorganisms are used in wastewater treatment to decompose organic matter. In some processes bacteria adhere to surfaces forming an extracellular matrix called biofilm. This project investigated biofilm development on rotating discs placed in a bioreactor. The biofilm was visualized by microscopy and evaluated by daily bioreactor monitoring. Three runs were completed using various reactor feeds and disc materials to study the effects on biofilm development. A fluorescence staining protocol using a lectin probe was developed to characterize the extracellular matrix.
Worcester Polytechnic Institute
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Chemistry and Biochemistry