Faculty Advisor

Wobbe, Kristin K.

Abstract

Most plants are capable of mounting resistance responses to various pathogen attacks. For a hypersensitive response (HR) to occur, a dominant or semi-dominant resistance (R) plant gene is required to recognize a dominant avirulence (Avr) factor of pathogen. Three types of Arabidopsis thaliana, Dijon-17 (Di-17), Dijon-3 (Di-3), and Columbia-0 (Col-0), are significant in understanding the genetics of Turnip crinkle virus (TCV) resistance. It has been shown that three genes are needed for successful resistance to TCV in A. thaliana: the dominant R gene HRT, the recessive gene rrt, and a third gene,TIP. Crosses of Di-17 and Di-3 plants, and crosses of Di-17 and Col-0 plants are being analyzed to determine phenotype upon mutant D4N TCV inoculation, and genotype of the F1 and F2 progeny. Using cleaved amplified polymorphic sequence (CAPS) markers, it is possible to determine the genotype of the progeny compared to the wild-type parents at the HRT and TIP loci. Although an HRT marker has already been identified, it is necessary to design primers specific for TIP gene amplification. Genetic and phenotypic analysis of the F2 generation was initiated.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2002

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Restricted-WPI community only

Advisor Department

Chemistry and Biochemistry

Advisor Program

Chemistry and Biochemistry

Share

COinS