Wobbe, Kristin K.
Most plants are capable of mounting resistance responses to various pathogen attacks. For a hypersensitive response (HR) to occur, a dominant or semi-dominant resistance (R) plant gene is required to recognize a dominant avirulence (Avr) factor of pathogen. Three types of Arabidopsis thaliana, Dijon-17 (Di-17), Dijon-3 (Di-3), and Columbia-0 (Col-0), are significant in understanding the genetics of Turnip crinkle virus (TCV) resistance. It has been shown that three genes are needed for successful resistance to TCV in A. thaliana: the dominant R gene HRT, the recessive gene rrt, and a third gene,TIP. Crosses of Di-17 and Di-3 plants, and crosses of Di-17 and Col-0 plants are being analyzed to determine phenotype upon mutant D4N TCV inoculation, and genotype of the F1 and F2 progeny. Using cleaved amplified polymorphic sequence (CAPS) markers, it is possible to determine the genotype of the progeny compared to the wild-type parents at the HRT and TIP loci. Although an HRT marker has already been identified, it is necessary to design primers specific for TIP gene amplification. Genetic and phenotypic analysis of the F2 generation was initiated.
Worcester Polytechnic Institute
Major Qualifying Project
Access to this report is limited to members of the WPI community. Please contact a project advisor or their department to request access
Restricted-WPI community only
Chemistry and Biochemistry