Adams, David S.
Neuroprotective peptides such as NMI-9236 are synthetic drugs designed for emergency stroke treatment. Prior research demonstrated that these peptides upregulate functional activator protein-1 (AP-1), which is a transcription factor thought to induce the gene expression of anti-oxidative enzymes. The purpose of this MQP was to investigate two aspects of these neurotrophic peptides. The first part was to determine if the upregulated AP-1 is capable of inducing trans-activation in a reporter plasmid. The second was to determine whether various peptides upregulate anti-oxidative enzymes as measured by Western blot analysis. The results indicate that AP-1 is indeed capable of trans-activation, and that anti-oxidative enzymes are upregulated in cell lines stimulated by various neurotrophic peptides.
Worcester Polytechnic Institute
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Biology and Biotechnology