University of Rochester
Of great interest to the pharmaceutical market, GPCR behavior is still not well understood. The interplay between ligand binding, receptor activation, and the resulting cellular response is not well categorized. Current assays to measure activation are indirect and rely on downstream activation of reporter genes. Successful in other GPCRs, a FRET-based direct indicator of activation was developed in Ste2p. Identification of a successful clone will allow further studies of GPCR behaviors, such as fractional occupancy. Pharmaceutical dosing of GPCR-targeted medicines relies on understanding the dynamic relationship of binding, activation, and output that impacts receptor behavior.
Worcester Polytechnic Institute
Major Qualifying Project
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Chemistry and Biochemistry
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