Faculty Advisor

Heilman, Destin

Abstract

Of great interest to the pharmaceutical market, GPCR behavior is still not well understood. The interplay between ligand binding, receptor activation, and the resulting cellular response is not well categorized. Current assays to measure activation are indirect and rely on downstream activation of reporter genes. Successful in other GPCRs, a FRET-based direct indicator of activation was developed in Ste2p. Identification of a successful clone will allow further studies of GPCR behaviors, such as fractional occupancy. Pharmaceutical dosing of GPCR-targeted medicines relies on understanding the dynamic relationship of binding, activation, and output that impacts receptor behavior.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2018

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Restricted-WPI community only

Advisor Department

Chemistry and Biochemistry

Advisor Program

Chemistry and Biochemistry

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