Current computational models for traumatic brain injury are limited by a lack of understanding of the changes in neuronal function following repeated strain, highlighting the need for large datasets characterizing neuronal response to stretch. To provide a platform capable of gathering these datasets, an apparatus to strain embedded neuronal samples and monitor the subsequent neural response optogenetically was developed. The apparatus enables loading and immobilization of live Caenorhabditis elegans specimen, delivers stretch stimulus to the embedded animals, and provides sufficient image resolution to analyze neuronal calcium activity fluorescently both pre- and post-stretch.
Worcester Polytechnic Institute
Major Qualifying Project
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